mesenchymal stem cells bmscs  (ATCC)

Journal: Biology

Article Title: Grape Pomace Polyphenolic Extract Promotes Osteogenic Differentiation in Human Mesenchymal Stem Cells Through Activation of RUNX2 and NRF2 Transcription Factors: A Potential Natural Strategy for Osteoporosis Prevention

doi: 10.3390/biology15090719

Figure Lengend Snippet: Effects of GPE on osteoblast differentiation of human MSCs. BMSCs and AdMSCs were cultured in basal medium (CTR) or osteogenic differentiation medium (OM) with or without GPE (1–10 µg GAE/mL) for up to 21 days, and matrix mineralization was analyzed by Alizarin Red S staining. Representative light microscopic images of Alizarin Red S staining in BMSCs ( A ) and AdMSCs ( B ) and quantitative analysis ( C ). Each experiment was performed in triplicate. All data are presented as mean ± standard deviation (SD). # p < 0.01 versus undifferentiated control cells (CTR); * p < 0.05 versus osteogenic differentiated cells (OM).

Article Snippet: Human bone marrow-derived mesenchymal stem cells (BMSCs) from healthy donors (ATCC-PCS-500-012) were purchased from ATCC (Milan, Italy) as well as mesenchymal stem cell basal medium (ATCC PCS500030) and mesenchymal stem cell growth kit for BMSC (ATCC PCS500041).

Techniques: Cell Culture, Staining, Standard Deviation, Control

Journal: Biology

Article Title: Grape Pomace Polyphenolic Extract Promotes Osteogenic Differentiation in Human Mesenchymal Stem Cells Through Activation of RUNX2 and NRF2 Transcription Factors: A Potential Natural Strategy for Osteoporosis Prevention

doi: 10.3390/biology15090719

Figure Lengend Snippet: Effects of GPE on the adipogenic differentiation of human MSCs. BMSCs and AdMSCs were cultured in basal medium (CTR) or adipogenic differentiation medium (AM) with or without GPE (1–10 µg GAE/mL) for up to 21 days, and lipid droplet formation was analyzed by Oil Red O (ORO) staining. Representative light microscopic images of ORO staining in BMSCs ( A ) and AdMSCs ( B ) and quantitative analysis ( C ). Each experiment was performed in triplicate. All data are presented as mean ± standard deviation (SD). # p < 0.01 versus undifferentiated control cells (CTR); * p < 0.05 and ** p < 0.01 versus adipogenic differentiated cells (AM).

Article Snippet: Human bone marrow-derived mesenchymal stem cells (BMSCs) from healthy donors (ATCC-PCS-500-012) were purchased from ATCC (Milan, Italy) as well as mesenchymal stem cell basal medium (ATCC PCS500030) and mesenchymal stem cell growth kit for BMSC (ATCC PCS500041).

Techniques: Cell Culture, Staining, Standard Deviation, Control

Journal: Biology

Article Title: Grape Pomace Polyphenolic Extract Promotes Osteogenic Differentiation in Human Mesenchymal Stem Cells Through Activation of RUNX2 and NRF2 Transcription Factors: A Potential Natural Strategy for Osteoporosis Prevention

doi: 10.3390/biology15090719

Figure Lengend Snippet: Effects of GPE on human mesenchymal stem viability. BMSC and AdMSC were exposed at increasing concentrations of GPE (1, 5, 10 and 25 µg GAE/mL) for 24 h and cell viability was evaluated by MTT assay. Data are shown as mean ± SD ( n = 5); * p < 0.05 versus untreated control cells (CTR).

Article Snippet: Human bone marrow-derived mesenchymal stem cells (BMSCs) from healthy donors (ATCC-PCS-500-012) were purchased from ATCC (Milan, Italy) as well as mesenchymal stem cell basal medium (ATCC PCS500030) and mesenchymal stem cell growth kit for BMSC (ATCC PCS500041).

Techniques: MTT Assay, Control

Journal: Scientific Reports

Article Title: Co-incorporation of Phosphorus and zinc into wollastonite ceramic granules synergically facilitating thin-walled structures regeneration

doi: 10.1038/s41598-026-44387-7

Figure Lengend Snippet: ( A ) The viability of BMSCs cultured on different concentrations of material(1/4, 1/8, 1/16 and 1/32)extract solution after 1, 3, and 7 days measured by CCK-8; ( B ) Expression of osteogenic proteins (LRP5, Runx-2,β-catenin and SP7) in BMSCs following a 7-day osteogenic incubation on different concentrations of material (1/8 and 1/16) extract solution; ( C ) Relative mRNA expression levels (normalized to β-actin) of osteogenesis-related genes (LRP5, OCN, BMP-2 and Runx-2) in BMSCs following a 7-day osteogenic incubation on different concentrations of material༈1/8 and 1/16༉extract solution; ( D ) the Alizarin Red S staining (ARS) performed to visually assess the formation of mineralized nodules; ( E ) Spread plate images of S. aureus seeding on the surface of corresponding plates with 1/16 concentrations of material extract solution. Quantitative statistics of colony count of the four groups materials against. All data are expressed as the mean ± SD. Different letters represent significant differences between groups at the same time point, n = 6; * p <0.05; ** p <0.01; *** p <0.001. Group: A:8P/6Zn-3:0, B:8P/6Zn-2:1, C:8P/6Zn-1:2, D: 8P/6Zn-0:3.

Article Snippet: The Bone Mesenchymal Stem Cells (BMSCs) were isolated from the femoral bone marrow of 4-week-old Sprague-Dawley rats.

Techniques: Cell Culture, CCK-8 Assay, Expressing, Incubation, Staining